Fig. 4

Recombinant mRNA expression in four different PT1-CHO cell lines: a as measured by qRT-PCR using heavy chain (HC) and light chain (LC) primers; and b as the percentage of HC/LC qRT-PCR values. qRT-PCR results were obtained by absolute quantification standard curve method and given as the average of n = 8 independent time point measurements done at several passages (P56 to P72). Two-way ANOVA was significant (***P < 0.0001); Mann – Whitney U test (two-tailed) for PT1-1 vs. PT1-7, PT1-30, or PT1-55) was also significant (***P = 0.0002). Data represent means and standard error of the means (SEM) of n = 8 independent measurements.