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Table 4 The kinetic parameters of the wild-type EstS and its mutant

From: Characterization of a cold-active esterase from Serratia sp. and improvement of thermostability by directed evolution

Enzyme K m (μM) K cat (s-1) K cat/K m (s-1 · μM-1)
WT 74.02 ± 1.9 2.339 × 103 ± 4.6 31.60 ± 3.1
1-D5 87.59 ± 2.4 2.501 × 103 ± 6.5 28.55 ± 3.7
  1. Data are given as mean values ± SD. All the assays were performed at the optimum pH and temperature