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Table 4 The kinetic parameters of the wild-type EstS and its mutant

From: Characterization of a cold-active esterase from Serratia sp. and improvement of thermostability by directed evolution

Enzyme

K m (μM)

K cat (s-1)

K cat/K m (s-1 · μM-1)

WT

74.02 ± 1.9

2.339 × 103 ± 4.6

31.60 ± 3.1

1-D5

87.59 ± 2.4

2.501 × 103 ± 6.5

28.55 ± 3.7

  1. Data are given as mean values ± SD. All the assays were performed at the optimum pH and temperature
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BMC Biotechnology

ISSN: 1472-6750

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