Table 2 Effect of organic solvents on the activity of Est WT and 1-D5
Organic solvent | Relative activity (%) | |||||
|---|---|---|---|---|---|---|
EstS | 1-D5 | |||||
10Â % (v/v) | 20Â % (v/v) | 30Â % (v/v) | 10Â % (v/v) | 20Â % (v/v) | 30Â % (v/v) | |
Control | 12.2 ± 0.5 U/ml | 10.9 ± 0.2 U/ml | 11.2 ± 0.3U/ml | 8.2 ± 1U/ml | 8.2 ± 1U/ml | 8.2 ± 1U/ml |
Isopropanol | 100.6 ± 4.1 | 12.0 ± 2.3 | 6.1 ± 5.7 | 66.8 ± 1.7 | 29.1 ± 6.7 | 14.6 ± 1.6 |
Acetone | 99.6 ± 1.2 | 103.9 ± 4.0 | 108.6 ± 2.4 | 46.2 ± 6.1 | 19.2 ± 2.1 | 11.6 ± 0.2 |
Methanol | 112.6 ± 4.6 | 46.5 ± 4.9 | 3.9 ± 0.3 | 79.6 ± 5.2 | 57.9 ± 4.5 | 33.0 ± 4.0 |
DMSO | 102.5 ± 5.8 | 119.8 ± 3.7 | 108.9 ± 0.3 | 90.2 ± 5.4 | 76.9 ± 7.0 | 40.5 ± 4.5 |
Ethanol | 121.4 ± 3.5 | 113.7 ± 1.9 | 110.5 ± 2.5 | 77 ± 9.7 | 44.6 ± 3.9 | 14.3 ± 2.2 |
N-butyl alcohol | ND | ND | ND | ND | ND | ND |
Ethylene glycol | 87.8 ± 2.2 | 83.7 ± 4.3 | 36.4 ± 5.6 | 90.9 ± 5.5 | 93.9 ± 5.9 | 90 ± 5.2 |
Acetonitrile | ND | ND | ND | ND | ND | ND |