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Table 2 Effect of organic solvents on the activity of Est WT and 1-D5

From: Characterization of a cold-active esterase from Serratia sp. and improvement of thermostability by directed evolution

Organic solvent Relative activity (%)
EstS 1-D5
10 % (v/v) 20 % (v/v) 30 % (v/v) 10 % (v/v) 20 % (v/v) 30 % (v/v)
Control 12.2 ± 0.5 U/ml 10.9 ± 0.2 U/ml 11.2 ± 0.3U/ml 8.2 ± 1U/ml 8.2 ± 1U/ml 8.2 ± 1U/ml
Isopropanol 100.6 ± 4.1 12.0 ± 2.3 6.1 ± 5.7 66.8 ± 1.7 29.1 ± 6.7 14.6 ± 1.6
Acetone 99.6 ± 1.2 103.9 ± 4.0 108.6 ± 2.4 46.2 ± 6.1 19.2 ± 2.1 11.6 ± 0.2
Methanol 112.6 ± 4.6 46.5 ± 4.9 3.9 ± 0.3 79.6 ± 5.2 57.9 ± 4.5 33.0 ± 4.0
DMSO 102.5 ± 5.8 119.8 ± 3.7 108.9 ± 0.3 90.2 ± 5.4 76.9 ± 7.0 40.5 ± 4.5
Ethanol 121.4 ± 3.5 113.7 ± 1.9 110.5 ± 2.5 77 ± 9.7 44.6 ± 3.9 14.3 ± 2.2
N-butyl alcohol ND ND ND ND ND ND
Ethylene glycol 87.8 ± 2.2 83.7 ± 4.3 36.4 ± 5.6 90.9 ± 5.5 93.9 ± 5.9 90 ± 5.2
Acetonitrile ND ND ND ND ND ND
  1. Data are given as mean values ± SD. The activity without any additives was defined as 100 %. ND means not detected