Skip to main content

Table 1 Effects of various reagents on the activity of EstS and mutant

From: Characterization of a cold-active esterase from Serratia sp. and improvement of thermostability by directed evolution

Reagents Relative activity (%)
EstS 1-D5
1 mM 5 mM 1 mM 5 mM
Control 9.9 ± 0.2 U/ml 8.6 ± 0.5 U/ml 8.1 ± 3.2 U/ml 8.1 ± 3.2 U/ml
Mg2+ 121.8 ± 1.3 106.5 ± 5.9 95.0 ± 4.4 98.3 ± 1.4
Sr2+ 102.0 ± 1.7 105.3 ± 2.3 94.2 ± 4.4 101.1 ± 1.1
Ba2+ 109.7 ± 5.7 107.6 ± 7.4 97.2 ± 3.1 103.2 ± 5.3
Zn2+ 83.1 ± 3.6 71.6 ± 4.1 39.9 ± 6.3 34.2 ± 4.6
Mn2+ 120.7 ± 4.0 114.9 ± 4.4 100.1 ± 7.3 106.2 ± 0.8
Cu2+ 87.6 ± 4.2 52.1 ± 4.0 97.0 ± 7.0 73.9 ± 3.6
Ca2+ 101.1 ± 1.3 106.2 ± 5.4 98.6 ± 3.5 101.4 ± 3.4
EDTA 96.3 ± 3.6 96.7 ± 1.0 99.1 ± 4.0 97.9 ± 2.6
PMSF 96.4 ± 3.5 89.8 ± 5.0 96.6 ± 4.7 54.8 ± 3.6
  1. Data are given as mean values ± SD. Activity was measured after adding 1 or 5 mM of various reagents into the reaction mixture. The activity without any additives was defined as 100 %