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Table 1 Hit rates (proportion of antigen recognizing clones) achieved from positive library pools in different HybriFree experiments

From: HybriFree: a robust and rapid method for the development of monoclonal antibodies from different host species

Source Library Antigen Pos./all (%) Comments
Chicken scFv Persephine 18/118 (15 %) After B-cell capturing as described here, the screening was done using E. coli based system
GFRα1 71/140 (50 %)
scFv-Fc hDNase 3/43 (7 %)  
hNGF 3/23 (13 %)  
HPV18 E2 7/93 (8 %) Full-length protein was used for immunization but the B-cell capturing and screening were performed using only C-terminal domain (one-third)
Mouse scFv Persephine 19/190 (10 %) After B-cell capturing as described here, the screening was done using E. coli based system
scFv-Fc Artemin 12/78 (15 %)  
hNGF 7/24 (29 %)  
Rabbit scFv-Fc hCD48 7/46 (15 %)  
HPV18 E2N-GST 13/93 (14 %)  
HPV18 E2C-GST 16/188 (9 %) Summarized results obtained from 2 rabbits
mCD48 80/186 (43 %)  
Ebola VLP 25/94 (27 %)  
Ebola GP 104/234 (44 %) Summarized results obtained from 3 experiments
IgG Ebola GP 11/46 (24 %)  
mCD48 55/94 (59 %) see section “Screening natural intact IgG molecules” for more details
hBDNF 43/125 (34 %)