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Table 1 Hit rates (proportion of antigen recognizing clones) achieved from positive library pools in different HybriFree experiments

From: HybriFree: a robust and rapid method for the development of monoclonal antibodies from different host species

Source

Library

Antigen

Pos./all (%)

Comments

Chicken

scFv

Persephine

18/118 (15 %)

After B-cell capturing as described here, the screening was done using E. coli based system

GFRα1

71/140 (50 %)

scFv-Fc

hDNase

3/43 (7 %)

 

hNGF

3/23 (13 %)

 

HPV18 E2

7/93 (8 %)

Full-length protein was used for immunization but the B-cell capturing and screening were performed using only C-terminal domain (one-third)

Mouse

scFv

Persephine

19/190 (10 %)

After B-cell capturing as described here, the screening was done using E. coli based system

scFv-Fc

Artemin

12/78 (15 %)

 

hNGF

7/24 (29 %)

 

Rabbit

scFv-Fc

hCD48

7/46 (15 %)

 

HPV18 E2N-GST

13/93 (14 %)

 

HPV18 E2C-GST

16/188 (9 %)

Summarized results obtained from 2 rabbits

mCD48

80/186 (43 %)

 

Ebola VLP

25/94 (27 %)

 

Ebola GP

104/234 (44 %)

Summarized results obtained from 3 experiments

IgG

Ebola GP

11/46 (24 %)

 

mCD48

55/94 (59 %)

see section “Screening natural intact IgG molecules” for more details

hBDNF

43/125 (34 %)