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Fig. 8 | BMC Biotechnology

Fig. 8

From: HybriFree: a robust and rapid method for the development of monoclonal antibodies from different host species

Fig. 8

a Applications of the HybriFree MAbs. a Anti-human Dnase I IgY antibodies were re-constructed from developed MAb sequences from chicken. These were produced in CHO cells and can be used for the detection of a ~35 kD hDNase I band by western blot assay (the expression vector producing non-relevant IgY is used as a negative control). b GP-positive Ebola VLPs MAb derived from a rabbit immunized with Ebola virus VLPs serves as a sensitive and selective reagent for the visualization of Ebola virus glycoprotein (GP) expression (green) in human cells by immunofluorescence. Nuclei are counterstained with DAPI (blue). c ELISA assay with 4 anti-GP MAbs (each represented by different color) using purified GP (filled circles), GP-positive Ebola VLPs (open circles) or GP-negative Ebola VLPs (Xs) as a target

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