Fig. 2

Amplification of VH and VL cDNA from spleen cells captured on an antigen-coated surface. cDNA was prepared from cells captured on the antigen-coated surface (+) and from control wells coated with BSA (−). a Splenocytes from chicken immunized with human DNase I protein. b Spleen homogenate from mouse isolated after immunization with human artemin protein. c Spleen homogenate from rabbit immunized with GST-E2C fusion protein. d Improvement of the VH and VL product yield by NaN₃. Chicken splenocytes were captured in capture medium with or without 0.1 % NaN₃, as indicated. Amplification from human β-actin cDNA was performed (209 bp product from spliced mRNA) as equal amounts of human cell lysate were used as a carrier during RNA isolation