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Fig. 1 | BMC Biotechnology

Fig. 1

From: HybriFree: a robust and rapid method for the development of monoclonal antibodies from different host species

Fig. 1

General workflow of the HybriFree method. The key steps include (STEP 1) the enrichment of source material by capturing cells producing specific antibodies; (STEP 2) the amplification of VH and VL cDNA; (STEP 3) construction of a combinatorial VH-VL library in scFv-Fc (or IgG) expression plasmid DNA format; and (STEP 4) screening proper VH-VL combinations after expression of MAbs in mammalian cells

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BMC Biotechnology

ISSN: 1472-6750

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