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Table 3 Primer sequences of the three single point mutations on AAV-DJ capsid

From: Single point mutation in adeno-associated viral vectors -DJ capsid leads to improvement for gene delivery in vivo

Residue

Sequence (5’ to 3’)

Nucleotide change

K137R

Wild-type primer sequence: TGAGGAAGCGGCTAAGACG

AAG → AGG

Mutant primer sequence: TGAGGAAGCGGCTAGGACG

T251A

Wild-type primer sequence: CCCTGCCCACCTACAACAAC

ACC → GCC

Mutant primer sequence: CCCTGCCCGCCTACAACAAC

S503A

Wild-type primer sequence: GTGAATACTCGTGGACTGGAG

TCG → GCG

Mutant primer sequence: GTGAATACGCGTGGACTGGAG

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BMC Biotechnology

ISSN: 1472-6750

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