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Table 2 Formula used for simple and complex osteogenic mediums

From: Dental follicle stem cells in bone regeneration on titanium implants

Simple osteogenic differentiation medium (OS) Complex osteogenic differentiation medium (OC)
• DMEM high glucose:F12 HAM(1:1)
• 10 % fetal calf serum
• Penicillin(100U/ml) + Streptomycin (100 μg/ml)
• 2 mM L-glutamine
• Non-essential aminoacids 1 %
• 10 mM β glicero-phosphate
• 50 μg ascorbic acid
• 10 nM dexamethasone
• DMEM glucose :F12 HAM(1:1)
• 10 % fetal calf serum
• Penicillin(100U/ml) + Streptomycin (100 μg/ml)
• 2 mM L-glutamine
• Non-essential aminoacids 1 %
• 10 mM β glicero-phosphate
• 50 μg ascorbic acid
• 10 nM dexamethasone
• 3 ng/ml BMP2
• 2 ng/ml TGF β1