Dye decolorization and detoxification potential of Ca-alginate beads immobilized manganese peroxidase

BMC Biotechnology

Table 2 Purification of extracellular MnP from G. lucidum IBL-05

Procedure	Total volume (mL)	Total protein (mg)	Total activity (UI)^a	Specific activity^b (UI mg⁻¹)	Purification^c (fold)
Crude extract	300	1302.3	215220	165.3	1
Ammonium Sulphate ppt.	22	43.9	13501.4	307.5	1.86
Dialysis	21	39.2	12559.9	320.4	1.94
DEAE-cellulose	12	6.37	6811.2	1069.3	6.47
Sephadex G-100	9	2.9	4599.1	1585.9	9.6

^aUI is defined as the amount of enzyme converting 1 μmol of substrate per minute.
^bSpecific activity = UI per mg protein.
^cPurification fold= Specific activity at given step/Specific activity of initial extract.

ISSN: 1472-6750