Skip to main content

Table 2 Purification of extracellular MnP from G. lucidum IBL-05

From: Dye decolorization and detoxification potential of Ca-alginate beads immobilized manganese peroxidase

Procedure Total volume (mL) Total protein (mg) Total activity (UI)a Specific activityb (UI mg−1) Purificationc (fold)
Crude extract 300 1302.3 215220 165.3 1
Ammonium Sulphate ppt. 22 43.9 13501.4 307.5 1.86
Dialysis 21 39.2 12559.9 320.4 1.94
DEAE-cellulose 12 6.37 6811.2 1069.3 6.47
Sephadex G-100 9 2.9 4599.1 1585.9 9.6
  1. aUI is defined as the amount of enzyme converting 1 μmol of substrate per minute.
  2. bSpecific activity = UI per mg protein.
  3. cPurification fold= Specific activity at given step/Specific activity of initial extract.