Fig. 4From: Dye decolorization and detoxification potential of Ca-alginate beads immobilized manganese peroxidaseDetermination of K m and V max by Lineweaver Burk Plot: Standard quartz (Si O 2) cuvettes of 1 mm path length were used to calculate the values of kinetic parameters. Both free and immobilized MnP were incubated for 15 min at 30 °C in sodium malonate buffer of pH 4.5 before carrying out standard enzyme assay protocol. Lineweaver‐Burk (Double reciprocal) plots were generated with Microsoft Excel Windows updated version 7 via nonlinear regression analysis using different concentrations (0.1-1.0 mM) of manganese sulphate as substrate at optimum pH and temperature conditionsBack to article page