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Fig. 4 | BMC Biotechnology

Fig. 4

From: Dye decolorization and detoxification potential of Ca-alginate beads immobilized manganese peroxidase

Fig. 4

Determination of K m and V max by Lineweaver Burk Plot: Standard quartz (Si O 2) cuvettes of 1 mm path length were used to calculate the values of kinetic parameters. Both free and immobilized MnP were incubated for 15 min at 30 °C in sodium malonate buffer of pH 4.5 before carrying out standard enzyme assay protocol. Lineweaver‐Burk (Double reciprocal) plots were generated with Microsoft Excel Windows updated version 7 via nonlinear regression analysis using different concentrations (0.1-1.0 mM) of manganese sulphate as substrate at optimum pH and temperature conditions

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