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Table 1 Determination of cell concentrations and dsRNA yields obtained from a single co-cultivation of RNase-deficient E. coli

From: Large-scale production and antiviral efficacy of multi-target double-stranded RNA for the prevention of white spot syndrome virus (WSSV) in shrimp

Production scale Type of process Medium Cell density (×109 CFU/mLa) dsRNAyield (μg/mLa) Incubation time (hr.)
Laboratory - LB 2.4 ± 0.2 2.6 ± 0.8 8
- TB 4.5 ± 0.1 6.2 ± 0.2 8
Large Batch TB 21.1 ± 2.9 3.4 ± 0.5 8
Fed-batch TB 36.2 ± 4.5 95.0 ± 21.5 30
  1. a1 mL of bacteria culture