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Table 1 Determination of cell concentrations and dsRNA yields obtained from a single co-cultivation of RNase-deficient E. coli

From: Large-scale production and antiviral efficacy of multi-target double-stranded RNA for the prevention of white spot syndrome virus (WSSV) in shrimp

Production scale

Type of process

Medium

Cell density (×109 CFU/mLa)

dsRNAyield (μg/mLa)

Incubation time (hr.)

Laboratory

-

LB

2.4 ± 0.2

2.6 ± 0.8

8

-

TB

4.5 ± 0.1

6.2 ± 0.2

8

Large

Batch

TB

21.1 ± 2.9

3.4 ± 0.5

8

Fed-batch

TB

36.2 ± 4.5

95.0 ± 21.5

30

  1. a1 mL of bacteria culture