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Fig. 1 | BMC Biotechnology

Fig. 1

From: Large-scale production and antiviral efficacy of multi-target double-stranded RNA for the prevention of white spot syndrome virus (WSSV) in shrimp

Fig. 1

a Identification of multi-WSSV dsRNA in a single-batch culture by RT-PCR. Lanes M, 2log marker; lanes 1–2, laboratory-scale production with LB and TB medium, respectively; lanes 3–4, large-scale production under batch and fed-batch processes, respectively; Lane -, negative control using DEP-C water as template. b Integrity analysis of individual single-targeted dsRNA from bacterial cells. E. coli HT115 expressed WSSV051 and VP28 dsRNA were subjected for dsRNA extraction. The respective dsRNAs were characterized by nuclease treatments using RNase A and RNase III for digestion of the ssRNA and dsRNA, respectively

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BMC Biotechnology

ISSN: 1472-6750

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