Fig. 4From: Plant expression and characterization of the transmission-blocking vaccine candidate PfGAP50Indirect immunofluorescence assays demonstrating the reactivity of PfGAP50 specific rabbit immune IgG against different stage P. falciparum parasites. IFAs were performed on schizonts, gametocytes and gametes, using PfGAP50 specific rabbit immune IgG or IgG purified from neutral rabbit serum (NRS) as a negative control. Mouse anti-Pfs25 and anti-PfMSP1 antibodies were used to co-label the sexual-stage (Pfs25) and blood stage (PfMSP1) parasites, respectively. Mouse antibodies were visualized with Alexa Fluor 488-conjugated secondary antibodies (green) and rabbit antibodies with Alexa Fluor 594-conjugated secondary antibodies (red). Bar = 5 μmBack to article page