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Fig. 2 | BMC Biotechnology

Fig. 2

From: Plant expression and characterization of the transmission-blocking vaccine candidate PfGAP50

Fig. 2

SDS-PAGE/immunoblot analysis of plant produced PfGAP50. a: Purification of plastid-targeted PfGAP50 (PfGAP50-cTPH). For purification, 7.0 g infiltrated leaf material were used. Reducing SDS-PAGE (left panel) and immunoblot (right panel). b: Purification of ER-retarded PfGAP50 (PfGAP50-ERH). For purification, 4.5 g infiltrated leaf material were used. Reducing SDS-PAGE (left panel) and immunoblot (right panel). M: Prestained protein marker (Page Ruler Fermentas), 1: 3 μL load (plant extract), 2: 3 μL flow-through, 3: 6 μL elution step 1 (10 mM imidazole), 4: 6 μL elution step 2 (100 mM imidazole), 5: 6 μL elution step 3 (250 mM imidazole). Western blot was detected with rabbit anti-His6 serum and alkaline phosphatase labeled goat anti rabbit serum. c: Plot of mean values and standard deviation of the yields for finally purified (E3) PfGAP50-cTPH and PfGAP50-ERH, determined by densitometric analysis (against BSA equivalents) of SDS-PAGE from three independent replicates (SDS-PAGE shown in additional file 1)

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