A novel quantification method for the total demethylation potential of aquatic sample extracts from Bohai Bay using the EGFP reporter gene

Table 2 Repeated TDQ experiment with cells treated with different doses of 5-AZA-CdR

Groups	Original 5-AZA-CdR (μM)	n	PFC (%)	RSD (%)	Calculated 5-AZA-CdR (μM)	RR (%)
A0	0.00000	5	2.20 ± 0.46	21.1	-	-
A1	0.00016	5	7.48 ± 0.81	10.8	0.000127	79.5
A2	0.00080	5	8.72 ± 1.09	12.5	0.000933	116.7
A3	0.00400	5	9.66 ± 1.51	15.7	0.003655	91.4
A4	0.02000	5	11.18 ± 1.47	13.1	0.025493	127.5

Note. PFC is the percentage of positive fluorescence cells of the total cells. RSD is the relative standard deviation. RR is the Recovery Rate of the 5-AZA-CdR calculated for the original 5-AZA-CdR. There are significant differences for ANOVA analysis of PFC between all groups barring groups A1 and A2 and groups A2 and A3

ISSN: 1472-6750