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Table 2 Repeated TDQ experiment with cells treated with different doses of 5-AZA-CdR

From: A novel quantification method for the total demethylation potential of aquatic sample extracts from Bohai Bay using the EGFP reporter gene

Groups Original 5-AZA-CdR (μM) n PFC (%) RSD (%) Calculated 5-AZA-CdR (μM) RR (%)
A0 0.00000 5 2.20 ± 0.46 21.1 - -
A1 0.00016 5 7.48 ± 0.81 10.8 0.000127 79.5
A2 0.00080 5 8.72 ± 1.09 12.5 0.000933 116.7
A3 0.00400 5 9.66 ± 1.51 15.7 0.003655 91.4
A4 0.02000 5 11.18 ± 1.47 13.1 0.025493 127.5
  1. Note. PFC is the percentage of positive fluorescence cells of the total cells. RSD is the relative standard deviation. RR is the Recovery Rate of the 5-AZA-CdR calculated for the original 5-AZA-CdR. There are significant differences for ANOVA analysis of PFC between all groups barring groups A1 and A2 and groups A2 and A3