Fig. 8From: Novel avian single-chain fragment variable (scFv) targets dietary gluten and related natural grain prolamins, toxic entities of celiac diseaseIgY competes with scFv for antigen binding sites. a. IgY competitively displaces scFv from PT-Gliadin coated plates. Competitive ELISA was performed with a 60 min pre-incubation with anti-PT-Gliadin IgY at indicated concentrations followed by addition of anti-PT-Gliadin-scFv at a concentration of ~ 0.04 [μg/ml] for 40 min. Detection was performed with anti penta-His antibody, as it detects specifically the His-tag labelled scFv but does not react with IgY. ELISA principle is described in Methods. Data shown are OD (450 nm) values and represent mean values (+/−SEM) of triplicates. b. IgY competitively displaces scFv from wheat flour digest coated plates. Competitive ELISA was performed as described in A and Methods. Data shown are OD (450 nm) values and represent mean values (+/−SEM) of triplicatesBack to article page