Fig. 6
Purified scFv detects PT-Gliadin and natural wheat flour digest. a. ScFv binds to PT-Gliadin in a concentration -dependent manner. Purified scFv was diluted in indicated concentrations and binding was assessed by ELISA as described in Methods. Data shown are OD (450 nm) values and represent mean values (+/− SEM) of triplicates. b. Purified scFv binds to natural wheat flour digest in a concentration -dependent manner. Purified scFv was diluted in indicated concentrations and binding was assessed by ELISA as described in Methods. Data shown are OD (450 nm) values and represent mean values (+/− SEM) of triplicates. c. ScFv binding reaches approximate equilibrium after 3 h incubation time. Two single chain concentrations (500 and 1000 ng/ml respectively) were incubated for indicated time and binding to (c). PT-Gliadin or (d). wheat flour digest was assessed by ELISA as described in Methods. Data shown represent OD (450 nm) values at different incubation times compared to the OD (450 nm) value after 3 h (ODx/OD3h *100). Mean values (+/− SEM) of triplicates are shown