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Fig. 5 | BMC Biotechnology

Fig. 5

From: Novel avian single-chain fragment variable (scFv) targets dietary gluten and related natural grain prolamins, toxic entities of celiac disease

Fig. 5

ELISA based testing of scFv candidates for reactivity to PT-Gliadin. Bacterial crude extracts expressing candidate scFvs were tested for antigen binding by ELISA as described in Methods. Samples were diluted 10-fold in TBS supplemented with 0.05 % Tween and 1 % BSA. Mock represents the negative control (crude extracts of bacteria transformed with empty vector). Data shown are OD (450 nm) levels and represent mean values (+/− SD) of duplicates

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