Fig. 4

Expression time course of scFv which is soluble expressed in E. coli BL21/ (DE3). Crude extracts (30 μg protein per lane) from indicated time points before/after induction with 1 mM IPTG were separated by SDS-PAGE and blotted on PVDF membranes. Western Blot was performed as described in Methods. The upper lane shows target protein expression, the lower lane (E. coli 60 kDa HSP60 protein) represents the loading control. Mock represents the negative control (lysates of bacteria transformed with empty vector). Experiment shown is representative for at least three repeated experiments