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Fig. 10 | BMC Biotechnology

Fig. 10

From: Novel avian single-chain fragment variable (scFv) targets dietary gluten and related natural grain prolamins, toxic entities of celiac disease

Fig. 10

ScFv detects gluten-rich grain digests in Western Blot. PT-Gliadin (10 μg per lane) or different flour digests (50 μg per lane) were separated under reducing conditions on a 12 % Bis-Tris Gel and blotted on activated PVDF membranes. Western Blot was performed as described in Methods, using purified scFv in a 1:3000 (~2.5 μg/ml) dilution. Control represents a blot treated in the same way, but where primary antibody was omitted. Experiment shown is representative for at least two repeated experiments

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