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Fig. 2 | BMC Biotechnology

Fig. 2

From: Development of a high yield expression and purification system for Domain I of Beta-2-glycoprotein I for the treatment of APS

Fig. 2

a SDS-PAGE gel comparing crude lysates of small scale expression of tagged Domain I fusion protein in LB and TB media before and after induction. Recombinant tagged DI fusion is indicated by an arrow and migrates at 12 kDa. Samples were lysed using triton/PBS and diluted in PBS (5x) and 20 μl was loaded onto an SDS PAGE Gel. The gel was then run, washed briefly in ddH2O and stained for 1 h with InstantBlue™ stain (Expedeon, UK). b & c Densitometric analysis was carried out on LB and TB samples on a small scale loaded in an identical way, gels were scanned, images converted to TIFF files and analysed. Values represent a calculation of intensity divided by area, OD denotes optical density of cultures at 600 nm at induction

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