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Fig. 3 | BMC Biotechnology

Fig. 3

From: Mouse Resistin (mRetn): cloning, expression and purification in Escherichia coli and the potential regulative effects on murine bone marrow hematopoiesis

Fig. 3

HPLC analysis results of purified rmRetn. a: RP-HPLC analysis of purified rmRetn was employed to detect sulphoxides, deamidates and/or impurities. Totally 28.5 μg rmRetn in 30 μl was loaded for analysis and two peaks were observed in the RP-HPLC spectrum, with the large one (1) identified as rmRetn and minor one (2) as other material. The purity by RP-HPLC was 97.6 %; b: SEC-HPLC was performed to detect possible polymers of rmRetn. Totally 47.5 μg rmRetn in 50 μl was loaded for analysis and only one rmRetn peak (1) was checked in SEC-HPLC spectrum and the purity of rmRetn was 100 %. Both spectra were detected at ultra violet (UV) wave length 280 nm and had been divided by blank control spectra respectively

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