Fig. 1

Upregulation of Retn in BM cells after ablation. a Normal Balb/c mice were injected with 5-Fu (300 mg/kg) or PBS as control at day 0. Total BM cell numbers per leg decreased remarkably post 5-Fu injection compared to the control (P < 0.001). 6 mice (3 males and 3 females) were individually investigated at each time point in 3 independent experiments. b Gene expression analysis in the Affymetrix oligonucleotide microarrays. Total mRNA of BM cells of mice was extracted at day 0, 3, 7, 11 and 14 post 5-fluorouracil injection and mixed thoroughly for genechip hybridization. 6 mice were utilized at every time point after chemotherapy, respectively. Microarray hybridizations were performed on gene chip Affymetrix mouse genome 430 2.0. The hybridization intensities of Retn were shown at 0, 3, 7, 11, 14 days after 5-Fu treatment. The mRNA level of mResistin reached the maximum of 16.3 fold of the normal level on the 7th day after chemotherapy. c Serum samples obtained from PB were assayed for Retn by ELISA. Samples of 3 mice at each time point were analyzed individually, and great increase was detected by day 7 (*P = 0.008) and 11 (*P = 0.003) after chemotherapy, compared with the basic concentration at day 0. All values were given as mean ± SD