Fig. 4From: Enhanced in vitro osteogenic differentiation of human fetal MSCs attached to 3D microcarriers versus harvested from 2D monolayersKinetics of gene expression, early and late markers, cell growth and calcium deposition during osteogenic differentiation of collagen I-coated plates seeded with microcarrier-harvested (3D MC-harv) or microcarrier-bound (3D MC-bound) S27 hfMSCs. a Osteogenic gene expression values normalized to Day 0 post-differentiation (*p < 0.05 and >2-fold difference). N = 3 biological replicates per condition. b Cell growth (cell density in 105 cells/cm2) (left) and calcium deposition per 106 cells (right), (*p < 0.05). N = 3 biological replicates per condition. Two-way repeated measures ANOVA with post-hoc Tukey correction was performed between 3D MC-harv, 2D MNL-harv and 3D MC-bound cells using Graphpad. Of the multiple comparisons performed, data representing a single comparison (3D MC-bound vs 3D MC-harv) is shown in this figureBack to article page