Fig. 3
Kinetics of gene expression, cell growth and calcium deposition during osteogenic differentiation of collagen I-coated plates seeded with microcarrier-harvested (3D MC-harv) or 2D monolayer-harvested (2D MNL-harv) S27 hfMSCs. a Osteogenic gene expression, early and late markers. Values normalized to Day 0 post-differentiation (*p < 0.05 and >2-fold difference). N = 3 biological replicates per condition. b Cell growth (cell density in 105 cells/cm2) (left) and calcium deposition per 106 cells (right), (*p < 0.05). N = 3 biological replicates per condition. Two-way repeated measures ANOVA with post-hoc Tukey correction was performed between 3D MC-harv and 2D MNL-harv and MC-bound cells using Graphpad. Of the multiple comparisons performed, data representing a single comparison (2D MNL-harv vs 3D MC-harv) is shown in this figure