Fig. 1From: Enhanced in vitro osteogenic differentiation of human fetal MSCs attached to 3D microcarriers versus harvested from 2D monolayers a Experimental Design. Human fetal mesenchymal stem cells (hfMSCs) from 2 different donors, denoted as S27 and S127 were cultured in growth media on Cytodex 3 microcarriers (3D MCs) in a spinner flask or as monolayers on tissue culture plastic flasks for 7Â days. Thereafter, cells on 3D MCs (3D MC-bound), or cells enzymatically harvested from MCs (3D MC-harv) were seeded onto a collagen I-coated plate and differentiated for 14Â days. Similarly cells expanded on uncoated (2D MNL-harv) or gelatin-coated (2D gelatin-MNL-harv) monolayer flasks were harvested and differentiated for 14Â days. b Microscopic images of S27 hfMSCs after 7Â days of expansion on Cytodex 3 microcarriersBack to article page