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Table 1 List of the primers and probes of the reference genes used in the RT-qPCR analysis

From: Transcriptome study and identification of potential marker genes related to the stable expression of recombinant proteins in CHO clones

Gene Name

Forward Primer (5′ → 3′)

Reverse Primer (5′ → 3′)

Probe (5′ → 3′)

Reference gene for copy number

Glucagon (Gluc)

ATTGCCAAACGCCACGAT

CCAAGCAATGAATTCCTTTGC

CTGAAGGGACCTTTACCA

Reference genes for gene expression

Actb

AGCCACGCTCGGTCAG

CATCCTGCGTCTGGACCT

CCGGGACCTGACAGACT

Gapdh

TCAACGGGAAGGCCATCAC

CCATTTGATGTTGGCGGGATC

TCGCTCCTGGAAGATG

Transgene

rDhfr

ATATGGGGATTGGCAAGAACG

CATTCTTTGGAAGTACTTGAACTCGTT

AGACCGACCCTGGC

  1. The reference genes were used for the copy number and gene expression calculations. All of the primers and probes were designed as TaqMan Gene Expression Assays labelled with FAM (3′) and MGB (5′). The sequence of the recombinant gene is confidential