Table 5 Comparison of the conserved amino acid positions following antigen selection
| Â | Pre-selection | Post-selection | ||
|---|---|---|---|---|
Heavy | Light (λ) | Heavy | Light (λ) | |
FW1 | 3 (19,14, 20) | 7 (3, 10, 11, 15, 19, 21, 23) | 21 (2–15, 17–22, 25–28) | 13 (3–7, 9, 11, 15, 16, 20–23) |
FW2 | 3 (36, 45, 48) | 2 (35, 47) | 9 (36–43, 45) | 6 (35, 40, 44, 45, 47, 48) |
FW3 | 4 (82, 82c, 90, 92) | 6 ( 61, 67, 73, 75, 82, 86) | 24 (66, 67, 70–72, 74, 76-82c, 86–92) | 23 (57–62, 64, 66, 70, 72–75, 77–79, 81–84, 86–88) |
FW4 | 0 | 3 (99, 102, 104) | 10 (103, 104, 106–113) | 10 (98, 99, 101–108) |
Total (FWs) | 10 | 18 | 62 | 51 |
CDR1 | 0 | 0 | 1 (34) | 4 (24, 25, 26, 33) |
CDR2 | 0 | 0 | 2 (51, 63) | 3 (54, 55, 56) |
CDR3 | 0 | 0 | 1 (101) | 0 |
Total (CDRs) | 0 | 0 | 3 | 7 |
Total (chain) | 10 | 18 | 65 | 58 |