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Table 3 CDR length distribution of the pre and post-selection sequences

From: Defining the complementarities between antibodies and haptens to refine our understanding and aid the prediction of a successful binding interaction

A

CDR length (percentage of representation within the pre-selection sequences)

CDR H1

CDR H2

CDR H3

CDR L1

CDR L2

CDR L3

Library 1

5 (100)

16 (97)

14 (14.5)

14 (65)

7 (100)

10 (62.5)

18 (2)

13 (11.8)

11 (20)

11 (28.8)

12 (10)

13 (14)

9 (2.5)

11 (8.18)

Library 2

5 (96.1)

16 (98.7)

11 (18)

14 (67)

7 (100)

10 (56.7)

6 (2.6)

15 (1.3)

14 (16)

13 (20)

11 (30)

7 (1.3)

13 (12)

11 (13.3)

12 (13.3)

9 (1.3)

 

9 (0)

Library 3

5 (100)

16 (100)

13 (16)

14 (52.6)

7 (100)

10 (67.4)

14 (15)

13 (24.2)

11 (21.1)

15 (13)

11 (20)

12 (7.4)

12 (8.7)

B

CDR length (percentage of representation within the post-selection clones)

CDR H1

CDR H2

CDR H3

CDR L1

CDR L2

CDR L3

Library 1

5 (100)

16 (100)

12 (89)

14 (89)

7 (100)

11 (89)

11 (11)

11 (11)

10 (11)

Library 2

5 (100)

16 (100)

11 (50)

13 (75)

7 (100)

9 (50)

9 (25)

14 (25)

10 (50)

13 (25)

Library 3

5 (100)

16 (100)

12 (100)

13 (100)

7 (100)

11 (100)

  1. The most prevalent CDRs lengths (number of amino acids) within each library in (A) Pre-selection clones and (B) Post-selection clones. The percentage of each specific CDR length within (A) the entire library population or (B) post-selection clones is in parenthesis