Defining the complementarities between antibodies and haptens to refine our understanding and aid the prediction of a successful binding interaction

Table 1 Summary of the sequence analysis strategy

	Immunised antigens	Library size	Sheep type	Number of pre-selection sequences		Number of post-selection clones
	Immunised antigens	Library size	Sheep type	H	L (λ)	Number of post-selection clones
Library 1	SQA and POR	4.83 x 10⁸	Cheviot cross	110	80	SQA (6) POR (3)
Library 2	COP	1.26 x 10⁸	Welsh Suffolk cross	77	30	COP (8)
Library 3	3-OXO-C12-HSL, 3-OH-C12-HSL, and N-acyl-C12-HSL	4.1 x 10⁷	Welsh Suffolk cross	196	95	HSL (6)

The analyzed sequences were obtained from three libraries. The pre and post-selection sequences included heavy (H) and lambda light (λ) chains. Pre-selection clones were randomly selected sequences during library construction process

ISSN: 1472-6750