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Table 4 Test of VitB2-UGM qPCR assay on Vitamin B2 80 % samples

From: Use of next generation sequencing data to develop a qPCR method for specific detection of EU-unauthorized genetically modified Bacillus subtilis overproducing riboflavin

Sample

Dilution

Average Cq-value

Sample vitamin B2 80 % (2014–3557)

1/10

13.9

1/100

17.4

Sample vitamin B2 80 % (2014–3558)

1/10

14.4

1/100

17.7

Sample vitamin B2 80 % (2014–3559)

1/10

14.3

1/100

17.6

NTC

na

N/A

  1. na not applicable, N/A no amplification
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BMC Biotechnology

ISSN: 1472-6750

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