Table 3 Limit of detection determination and repeatability of Cq-values of the VitB2-UGM qPCR assay
Estimated copy number | Mean Cq values ± SD | Positive signals (%) | RSDr | r |
|---|---|---|---|---|
10 | 31.99 ± 0.73 | 100 | 2.3 | 2 |
5 | 33.01 ± 0.47 | 100 | 1.4 | 1.3 |
2 | 34.86 ± 1.15 | 100 | 3.3 | 3.2 |
1 | 35.71 ± 1.07 | 94.4 | na | na |
0.1 | 36.41 ± 0.09 | 22.2 | na | na |
DBC (negative control) | N/A | 0 | na | na |