Table 2 Selectivity assessment of the VitB2-UGM qPCR assay for detection of the GM-Bacillus subtilis 2014–3557 overproducing riboflavin

Bacillus subtillis GM (2014–3557)

SCL

2014–3557

22.28

Bacillus subtillis GM (2014–3558)

SCL

2014–3558

26.10

Bacillus subtillis GM (2014–3559)

SCL

2014–3559

25.08

Bacillus subtillis subtilis (24 isolates)

UCL

^a

N/A

Bacillus cereus

IPH-FP

ATCC 14579

N/A

Bacillus lentus

IPH-FP

TIAC 0101

N/A

Bacillus licheniformis

IPH-FP

TIAC 0102

N/A

Bacillus sphaericus

IPH-FP

TIAC 0104

N/A

Bacillus thuringiensis

IPH-FP

TIAC 0096

N/A

Bacillus mycoides

IPH-FP

TIAC 0097

N/A

Bacillus circulans

IPH-FP

TIAC 0100

N/A

Escherichia coli

IPH-FP

ATCC 25922

N/A

Shigella boydii 10

Salm -NRC

12–2582

N/A

Aeromonas hydrophila

IPH-QML

6688 (M/2862)EEQ 2003/2

N/A

E. coli O145:H28

IPH-FP

TIAC 1360

N/A

Campylobacter jejuni

IPH-FP

ATCC 33291

N/A

Citrobacter freundii

IPH-FP

TIAC 0554

N/A

Enterobacter aerogenes

IPH-QML

3778 (M/317-M/3785) EEQ 2002/3

N/A

Streptococcus pyrogenes

IPH-QML

ATCC 19615

N/A

Hafnia alvei

IPH-QML

7186

N/A

Klebsiella pneumoniae

IPH-FP

TIAC 0446

N/A

Listeria monocytogenes

List- NRC

ATCC 51772

N/A

Proteus mirabilis

IPH-FP

TIAC 0726

N/A

Providencia rettgeri

IPH-QML

1521 (M/831) EEQ 1999/2

N/A

Pseudomonas aeruginosa

IPH-FP

LMG 6395

N/A

Salmonella enterica enterica Enteritidis

Salm-NRC

H,VI,6,32

N/A

Salmonella enterica enterica Typhimurium

Salm-NRC

H,II,13,13

N/A

Serratia marcescens

IPH-QML

7015

N/A

Staphylococcus aureus

IPH-FP

ATCC 25923

N/A

Vibrio parahaemolyticus

IPH-FP

TIAC 0610

N/A

Yersinia enterocolitica

IPH-FP

LMG 15558

N/A

NTC

N/A

CTD

N/A