Table 1 Primer pair sequences, position on contig 19, concentration and amplicon size for VitB2-UGM qPCR assay
Target | Primer pair name | Primer name | Position on contig 19 | Primer sequence, 5'->3'- | Primer/probe concentration | Amplicon | Origin pair size |
|---|---|---|---|---|---|---|---|
Contig00019 from Bacillus subtilis GM overproducing riboflavin 2014–3557 [Genbank:JYFL01000019.1] | VitB2-UGM | VitB2-UGM-F | 1,399–1,420 | GAT ACCAAACGAAAT GGGACAT | 250 nM | 128 bp | this study |
VitB2-UGM-R | 1,468–1,494 | TTCAG CGT AACAGACAT AATTTTT | 250 nM | this study | |||
VitB2-UGM-P | 1,503–1,526 | 1 ICCGIGIAGGAAI 1 1 1 IAGAGIAGAA | 100 nM | this study |