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Fig. 1 | BMC Biotechnology

Fig. 1

From: Use of next generation sequencing data to develop a qPCR method for specific detection of EU-unauthorized genetically modified Bacillus subtilis overproducing riboflavin

Fig. 1

Amplicon, primers and probe sequences of the VitB2-UGM qPCR assay, position of the junction GM-plasmid-insert. Green: similarity with plasmid pSM19035 and pBT233, light blue: “insert” – similarity with Bacillus subtilis subsp. subtilis str. 168 sequences (including riboflavin biosynthesis genes – in bold); striped blue box: gap of 37 basepairs between Contig00019 and Contig00022, sequence obtained by PCR followed by sequencing; Underlined: primers of the VitB2-UGM qPCR assay, bold: probe of the VitB2-UGM qPCR assay; the size and orientation of the contigs has been indicated at the top, as also the position of the junctions in the respective contigs; Genes located on the respective contigs are indicated with green and light blue arrows using annotation of B. subtilis strain 2014–3557, [Genbank: JYFL01000000] – genes encoding following proteins: bin3 - putative transposon Tn552 DNA-invertase; hyp – hypothetical protein; repS - protein RepS; copR - plasmid copy control protein CopR; KIX80113.1: accumulation-associated protein; hyp - hypothetical protein; tcpC - conjugative transposon protein TcpC; ypuI_2 - part of putative protein YpuI_2; scpB - segregation and condensation protein B; scpA-1 - segregation and condensation protein A; ypuF - putative protein YpuF; ribT_1 - protein RibT; ribH_1 - 6,7-dimethyl-8-ribityllumazine synthase; ribBA_1 = GTP cyclohydrolase-2; ribE_1 - riboflavin synthase; ribD_1–5-amino-6-(5-phosphoribosylamino)uracil reductase; ypuD - putative protein YpuD; sipS - signal peptidase I S; ypzJ - putative protein YpzJ; hyp - hypothetical protein; ypuB - putative protein YpuB; ypzD - spore germination protein-like protein YpzD; hyp - hypothetical protein; hyp - hypothetical protein; KIX80145.1 - N-acetylmuramoyl-L-alanine amidase domain-containing protein; hyp - hypothetical protein; hyp – hypothetical protein

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