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Table 4 Effects of metal ions and chemical reagents on the PulN activity

From: A novel cold-adapted type I pullulanase of Paenibacillus polymyxa Nws-pp2: in vivo functional expression and biochemical characterization of glucans hydrolyzates analysis

Reagents

Concentration

Relative activity (%)

None

-

100

EDTA

5 mM

102.1 ± 0.2

SDS

1 mM

12.3 ± 0.8

TritonX-100

0.1 %

99.8 ± 1.1

 

1 %

99.4 ± 0.2

Urea

2 M

71.2 ± 0.4

 

4 M

34.8 ± 2.3

2-Mercaptoethanol

5 mM

132.7 ± 1.9

 

10 mM

167.3 ± 4.0

DTT

10 mM

154.4 ± 1.5

α-cyclodextrin

0.1 %

77.8 ± 0.8

  1. The data represent the mean of three experimental repeats with SD of ≤ 5 %