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Table 4 Effects of metal ions and chemical reagents on the PulN activity

From: A novel cold-adapted type I pullulanase of Paenibacillus polymyxa Nws-pp2: in vivo functional expression and biochemical characterization of glucans hydrolyzates analysis

Reagents Concentration Relative activity (%)
None - 100
EDTA 5 mM 102.1 ± 0.2
SDS 1 mM 12.3 ± 0.8
TritonX-100 0.1 % 99.8 ± 1.1
  1 % 99.4 ± 0.2
Urea 2 M 71.2 ± 0.4
  4 M 34.8 ± 2.3
2-Mercaptoethanol 5 mM 132.7 ± 1.9
  10 mM 167.3 ± 4.0
DTT 10 mM 154.4 ± 1.5
α-cyclodextrin 0.1 % 77.8 ± 0.8
  1. The data represent the mean of three experimental repeats with SD of ≤ 5 %