Reagents | Concentration | Relative activity (%) |
---|---|---|
None | - | 100 |
EDTA | 5 mM | 102.1 ± 0.2 |
SDS | 1 mM | 12.3 ± 0.8 |
TritonX-100 | 0.1 % | 99.8 ± 1.1 |
 | 1 % | 99.4 ± 0.2 |
Urea | 2 M | 71.2 ± 0.4 |
 | 4 M | 34.8 ± 2.3 |
2-Mercaptoethanol | 5 mM | 132.7 ± 1.9 |
 | 10 mM | 167.3 ± 4.0 |
DTT | 10 mM | 154.4 ± 1.5 |
α-cyclodextrin | 0.1 % | 77.8 ± 0.8 |