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Table 2 Purification of PulN by Ni-NTA affinity chromatography

From: A novel cold-adapted type I pullulanase of Paenibacillus polymyxa Nws-pp2: in vivo functional expression and biochemical characterization of glucans hydrolyzates analysis

Purification step Total activity (U) Total protein (mg) Specific activity (U/mg) Yield (%) Purification fold
Crude extract 292.05 77.26 3.78 100.00 1.00
Ni-NTA 171.41 10.60 16.17 58.7 4.28
  1. The crude enzyme was obtained from 45 ml culture and 10 ml purified enzyme was obtained.