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Table 2 Purification of PulN by Ni-NTA affinity chromatography

From: A novel cold-adapted type I pullulanase of Paenibacillus polymyxa Nws-pp2: in vivo functional expression and biochemical characterization of glucans hydrolyzates analysis

Purification step

Total activity (U)

Total protein (mg)

Specific activity (U/mg)

Yield (%)

Purification fold

Crude extract

292.05

77.26

3.78

100.00

1.00

Ni-NTA

171.41

10.60

16.17

58.7

4.28

  1. The crude enzyme was obtained from 45 ml culture and 10 ml purified enzyme was obtained.