From: A novel cold-adapted type I pullulanase of Paenibacillus polymyxa Nws-pp2: in vivo functional expression and biochemical characterization of glucans hydrolyzates analysis
Purification step
Total activity (U)
Total protein (mg)
Specific activity (U/mg)
Yield (%)
Purification fold
Crude extract
292.05
77.26
3.78
100.00
1.00
Ni-NTA
171.41
10.60
16.17
58.7
4.28