A novel cold-adapted type I pullulanase of Paenibacillus polymyxa Nws-pp2: in vivo functional expression and biochemical characterization of glucans hydrolyzates analysis

Table 1 Pairwise similarity between the type I pullulanase amino acid sequences

Similarity value (%) for pullulanase sequence from:
Pullulanase sequence from:	P.polymyxa Nws-pp2	C.saccharolyticus	A.gottschalkii	Bacillus sp. CICIM 263	P.mucilaginosus	F.pennavorans Ven5	T.neapolitana
Paenibacillus polymyxa Nws-pp2	100	37.3	47.6	38.5	41.6	39.1	38.9
Caldicellulosiruptor saccharolyticus		100	43.2	36.8	40.0	37.3	41.1
Anaerobranca gottschalkii			100	41.3	44.4	43.8	44.4
Bacillus sp. CICIM 263				100	52.7	39.8	39.6
Paenibacillus mucilaginosus					100	41.5	42.6
Fervidobacterium pennavorans Ven5						100	68.0
Thermotoga neapolitana							100

The sequences are from the following sources: P. polymyxa Nws-pp2 (this study; GenBank accession no. KJ740392); C. saccharolyticus (GenBank accession no. L39876); A. gottschalkii (GenBank accession no. AY541591); Bacillus sp. CICIM 263 (GenBank accession no. AGA03915); P. mucilaginosus (GenBank accession no. YP004645603); F. pennavorans Ven5 (GenBank accession no. AF096862); T. neapolitana (GenBank accession no. FJ716701)

ISSN: 1472-6750