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Fig. 4 | BMC Biotechnology

Fig. 4

From: A novel cold-adapted type I pullulanase of Paenibacillus polymyxa Nws-pp2: in vivo functional expression and biochemical characterization of glucans hydrolyzates analysis

Fig. 4

Characterization of recombinant PulN. a Effect of pH on PulN activity at 35 °C in buffers ranging from pH 4.0-10.0. b Effect of pH on the stability of PulN activity. After incubation at 35 °C for 90 min in buffers ranging from pH 4.0-10.0 and the residual activity was measured at pH 6.0. c Effect of temperature on PulN activity assayed at pH 6.0. d Thermostability of PulN. The enzyme was preincubated at 35 °C (□), 40 °C (■), 45 °C (○), 50 °C (●) at pH 6.0. After various time intervals, samples were withdrawn and the residual activity was measured at 35 °C. The statistical tool Origin 8.0 was used to analyze the enzymatic properties data. Each value represents the mean ± SD of triplicate

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BMC Biotechnology

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