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Fig. 3 | BMC Biotechnology

Fig. 3

From: A novel cold-adapted type I pullulanase of Paenibacillus polymyxa Nws-pp2: in vivo functional expression and biochemical characterization of glucans hydrolyzates analysis

Fig. 3

SDS-PAGE of the recombinant protein in E. coli. a SDS-PAGE of recombinant PulN in different pET plasmid (induction temperature: 37 °C, induction time 24 h, IPTG concentration: 0.5 mM). Lane M: protein molecular weight markers; Lane 1: supernatant without inducer (BL21-pET-28-pulN); lane 2-3: precipitation and supernatant; lane 4: supernatant without inducer (BL21-pET-32-pulN); lane 5-6: precipitation and supernatant; lane 7: supernatant without inducer (BL21-pET-42-pulN); lane 8-9: precipitation and supernatant. b SDS-PAGE of the purified PulN. c Recombinant PulN production in BL21-pET-28-pulN with different induction temperatures (IPTG concentration: 0.1 mM, induction time 24 h). lane 1-2: supernatant and precipitation at 20 °C; lane 3-4: supernatant and precipitation at at 25 °C; lane 5-6: supernatant and precipitation at 30 °C; lane 7-8: supernatant and precipitation at at 37 °C. The histogram shows the comparison of pullulanase activity at different induction temperature. d Recombinant PulN production in BL21-pET-28-pulN with different induction time (IPTG concentration: 0.1 mM, induction temperature 20 °C). lane 1: supernatant of empty vector, lane 2: supernatant of BL21-pET-28-pulN without inducer; lane 3-8: supernatant of recombination strain after 2 h, 4 h, 8 h, 16 h, 24 h, 32 h induction (BL21-pET-28-pulN). The histogram shows the comparison of pullulanase activity after different induction time

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BMC Biotechnology

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