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Table 2 Purification of LipM from Escherichia coli BL21 (DE3)a

From: A novel eurythermic and thermostale lipase LipM from Pseudomonas moraviensis M9 and its application in the partial hydrolysis of algal oil

 

Concentration (mg/ml)

Volume (ml)

Total protein (mg)

Specific activity (U/mg)

Purification fold

Recovery (%)

Dialysis

1.02

50

50.9

60.6

1

100

LipM

0.44

20

8.8

141.73

2.34

40.43

  1. aLipase activity was determined with p-nitrophenyl caprylate as substrate