Primers | Function | Sequence (5'-3') |
---|---|---|
T5 | Degenerate primer | ACAATGTGCTGAACATNGGNTAYGARA |
T3 | Degenerate primer | GAAGGCGCCGCTGAANANNWANGTNTT |
lipM9-5-1 | Upstream genome walking | GACGCTTCCGCCAGGTTG |
lipM9-5-2 | Upstream genome walking | AGCCGTGAGCCGACCAGTTC |
lipM9-5-3 | Upstream genome walking | CCTGGTGTCCGTCGTGCTTG |
lipM9-3-1 | Downstream genome walking | GCTGTACGTGCGTGATGCCTAT |
lipM9-3-2 | Downstream genome walking | GGTGGGGGAGCAAGGAGGT |
lipM9-3-3 | Downstream genome walking | ACGGCAACACACTGGCAGC |
lipM9-F | Complete genetic sequence | ATGGGAYTGTTCGATTACARAAAYGCCGA |
lipM9-R | Complete genetic sequence | TYASGCRAASRYGAWRCYYGAAKCCGACAGG |
lipM9-NF | Expression vector construction | CTCATATGGGATTGTTCGATTAC |
lipM9-XR | Expression vector construction | CTCTCGAGCGCAAACATGATACTT |