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Fig. 7 | BMC Biotechnology

Fig. 7

From: A novel eurythermic and thermostale lipase LipM from Pseudomonas moraviensis M9 and its application in the partial hydrolysis of algal oil

Fig. 7

Selective hydrolysis of algal oil by LipM. a The gas chromatograph-mass spectrometry (GC–MS) analysis of raw algal oil showed kinds of fatty acid methyl esters. C14:0, methyl tetradecanoate; C16:0, hexadecanoic acid, methyl ester; C16:1, 9-hexadecenoic acid, methyl ester; C18:0, methyl stearate; C18:1, 9-Octadecenoic acid, methyl ester; C18:2, 9,12-Octadecadienoic acid, methyl ester; C20:5, 5,8,11,14, 17-Eicosapentaenoic acid, methyl ester; C22:5, 4,7,10,13,16-docosapentaenoate, methyl ester; C22:6, 4,7,10,13,16,19-docosahexaenoate, methyl ester; b The GC–MS analysis of algal oil showed kinds of fatty acid methyl esters after hydrolyzing for 3 h. c Thin layer chromatography (TLC) analysis after hydrolysis algal oil of LipM. 1:TGA (standard sample); 2:DGA (standard sample); 3: oleic acid (standard sample); 4:raw algal oil; 5–8: algal oil was hydrolyzed to remained partially triacylglycerols (TAGs), produce corresponding diacylglycerols (DAGs) and free fatty acids (FFAs) at 1 h, 2 h, 3 h and 4 h. d According to the analysis of HPLC, the relative contents of produced diacylglycerols (DAGs, ■) after hydrolyzing different time

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BMC Biotechnology

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