Fig. 4

SDS-PAGE analysis of the purified LipM. a 1: inclusion bodies; 2: inclusion bodies solubilized in solubilization buffer; b 1: purified LipM; M1: unstained protein molecular weight markers (Fermentas, SM0431); c Western blot analysis of the purified LipM with an anti-His tag antibody. M2: prestained protein molecular weight marker (Fermentas, SM0671); 1: LipM combined anti-His tag primary antibody and horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG second antibody; d Predicted lables (A-E) identify the peptide fragments of LipM whose molecular weights correspond to those of the peaks shown in the peptide mass fingerprint; e Peptide mass fingerprint generated by MALDI-TOF mass spectrometry of the purified LipM (x-axis, m/z ratio; y-axis, species abundance in terms of percent signal intensity)