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Table 1 I-SceI and ZFN activity at the site-specific integration locus

From: Gene stacking in plant cell using recombinases for gene integration and nucleases for marker gene deletion

Lines Nuclease Expression system1 Total # Perfect deletion2 Short indels3 Large indels4 No change5
UI I-SceI Constitutive 72 14 7 25 26
UZ ZFN Constitutive 68 4 5 38 21
HI I-SceI Heat-shock 7 3 - - 4
HZ ZFN Heat-shock 23 - - 13 10
  1. 1Strong constitutive (Ubi promoter) or heat-shock (HS) expression
  2. 2 < 15 bp indel at the target without the loss of sequences immediately adjacent to the nuclease target
  3. 350–200 bp insertion or deletion
  4. 4No amplicons obtained in PCR
  5. 5No DSB-repair as indicated by PCR and/or sequencing
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BMC Biotechnology

ISSN: 1472-6750

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