Table 1 Comparison of the Ca2+-aequorin assay and I−-YFPQL assay
From: A new high-throughput screening-compatible gap junctional intercellular communication assay
Ca2+-aequorin assay [18] | I−-YFPQL assay (this report) | |
|---|---|---|
Signal | Luminescence | Fluorescence |
Reagent needed for assay | Coelenterazine | None |
Assay time (sec per well) | 40 | 10 Longer assay time increases Z’ factor. |
Measuring physiologic event? | Yes (Ca2+) | No (I−) |
Sources of false positives | ● Adrenergic α1 receptor or TrpV1 channel in donor cells | ● SLC26A4 |
● Any target affecting cellular calcium | ● Any target increasing cellular permeability to I− | |
● Aequorin | ● YFPQL |