Fig. 2
From: A new high-throughput screening-compatible gap junctional intercellular communication assay
Fluorescence image of I−-YFP GJIC assay in LN215 cells. The LN215-YFPQL cells combined with wild-type LN215 (a) or LN215-I- (b) cells were plated on a 96-well plate. After 24 h of incubation, the culture media were replaced with 100 μL of C-solution. Phase contrast and fluorescence images were collected before and 1 min after adding 100 μL of I-solution. For the composition of the C- and I-solutions, see the I−-YFPQL GJIC assay in the Methods section