Fig. 1
From: A new high-throughput screening-compatible gap junctional intercellular communication assay
The scheme of I−-YFPQL GJIC assay. Black and yellow rectangles represent donor cells expressing an iodide transporter, SLC26A4, and acceptor cells with YFPQL. Blue and red parallel lines indicate gap junctions and iodide transporters, respectively. Acceptor cells were impermeable to iodide. Added iodide could only reach acceptor cells by passing through the iodide transporter in the donor cells and then through the gap junction between donor and acceptor cells. The YFPQL in acceptor cells was quenched by the iodide